African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Effects of different culture conditions (photoautotrophic, photomixotrophic) and the auxin indole-butyric acid on the in vitro acclimatization of papaya (Carica papaya L. var. Red Maradol) plants using zeolite as support

Laisyn Posada Perez
  • Laisyn Posada Perez
  • Instituto de Biotecnologia de las Plantas. Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara. Cuba.
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Yenny Padron Montesinos
  • Yenny Padron Montesinos
  • Instituto de Biotecnologia de las Plantas. Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara. Cuba.
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Justo Gonzalez Olmedo
  • Justo Gonzalez Olmedo
  • Centro de Bioplantas. Universidad de Ciego de Avila, Carretera a Moron km 9, Ciego de Ávila, Cuba.
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Romelio Rodríguez Sanchez
  • Romelio Rodríguez Sanchez
  • Centro de Bioplantas. Universidad de Ciego de Avila, Carretera a Moron km 9, Ciego de Ávila, Cuba.
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Osvaldo Norman Montenegro
  • Osvaldo Norman Montenegro
  • Centro de Bioactivos Químicos. Universidad Central “Marta Abreu” de Las Villas, Carretera a Camajuaní Km 5 ½. Santa Clara, Villa Clara, Cuba.
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Raul Barbón Rodriguez
  • Raul Barbón Rodriguez
  • Instituto de Biotecnologia de las Plantas. Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara. Cuba.
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Ortelio Hurtado Ribalta
  • Ortelio Hurtado Ribalta
  • Instituto de Biotecnologia de las Plantas. Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara. Cuba.
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Rene Carlos Rodriguez Escriba
  • Rene Carlos Rodriguez Escriba
  • Centro de Bioplantas. Universidad de Ciego de Avila, Carretera a Moron km 9, Ciego de Ávila, Cuba.
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Dion Daniels
  • Dion Daniels
  • University of Belize, Hummingbird Avenue, Belmopan, Cayo District, Belize, Central America.
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Rafael Gómez-Kosky*
  • Rafael Gómez-Kosky*
  • Instituto de Biotecnologia de las Plantas. Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara. Cuba.
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  •  Received: 23 June 2015
  •  Accepted: 31 August 2015
  •  Published: 02 September 2015

Abstract

Plant regeneration of papaya via organogenesis and somatic embryogenesis has been successful; however, the biggest problem of in vitro culture of this species is the acclimatization of regenerated plants, where over 70% of the plants are lost before being planted in the field. Decreasing the relative humidity inside the culture vessel and thus increasing the ventilation, appears to have a greater effect on the adaptation of papaya plants, strengthening the function of the stomata and with this, allowing better control of water loss from the leaves. The aim of this study was to determine the effects of different concentrations of sucrose and indole-butyric acid (IBA) on rooting and in vitro acclimatization of plants using sterile zeolite as support and culture vessels with increased ventilation. Three concentrations of sucrose (0, 10 and 20 g L-1) were studied with and without auxin and as the control treatment, the rooting culture medium with agar during 17, 27 and 37 culture days. The highest percentage of rooting was recorded at 37 culture days in the treatment without sucrose and IBA with 80.0% and zeolite as support. The best photosynthetic values were achieved when in vitro shoots were grown in culture medium with auxin and different concentrations of sucrose, even though they were also high in the treatment without the presence of IBA and without sucrose at 17 days of culture. The combined effect of the zeolite, auxin (IBA), without sucrose in the culture medium and increased ventilation allowed photoautotrophic culture conditions which had effect of the increasing plant survival under ex vitro acclimatization conditions.

Key words: Carica papaya, photosynthesis, roots formation.

Abbreviation

IBA, Indole-butyric acid; BAP, 6-benzyl aminopurine; NAA, naphthaleneacetic acid