African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Full Length Research Paper

Induction of mutations in Browallia speciosa using sodium azide and identification of the genetic variation by peroxidase isozyme

El-Mokadem, Hoda E.
  • El-Mokadem, Hoda E.
  • Department of Floriculture, Ornamental Horticulture and Garden design, Faculty of Agriculture, Alex. University, Alexandria, Egypt
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Mostafa, Gehan G.
  • Mostafa, Gehan G.
  • Department of Horticulture (Ornamental Plants), Faculty of Agriculture, South Valley University Qena, Egypt
  • Google Scholar


  •  Received: 31 October 2013
  •  Accepted: 12 December 2013
  •  Published: 01 January 2014

Abstract

Plants of Browallia speciosa were treated with different concentrations of sodium azide (0, 200, 400, 600 and 800 ppm) as a soil drench. The concentration of 800 ppm had simulative effect on the most studied traits. It increased the number of branches and leaves, chlorophyll content, fresh weights of vegetative growth and roots, dry weights of vegetative growth and roots and root length in the M1 (2011/ 2012) and  M2 (2012/ 2013). All the concentrations of sodium azide produced changes in the flower colour, flower shape and leaf form in both generations. Peroxidase isozyme pattern indicated that seven loci produced of peroxidase are shown in B. speciosa.  In addition, different peroxidase profiles among mutants were found. The phylogenetic tree indicated that mutant 4 was the most genetically distinct mutant from the control followed by mutant 3.

 

Key words: Browallia speciosa, mutation, sodium azide, isozyme.