African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Confirmation of antibodies against L-tryptophan-like epitope in human African trypanosomosis serological diagnostic

Silla Semballa
  • Silla Semballa
  • Université de Bordeaux, IRD, UMR 177 «INTERTRYP», 33076 Bordeaux France.
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Romaric Nzoumbou-Boko
  • Romaric Nzoumbou-Boko
  • Université de Bordeaux, IRD, UMR 177 «INTERTRYP», 33076 Bordeaux France.
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Philippe Holzmuller
  • Philippe Holzmuller
  • CIRAD UMR Contrôle des maladies exotiques et émergentes (CMAEE), TA-A15/G, Campus International de Baillarguet, 34398, Montpellier Cedex, France.
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Paul Chuchana
  • Paul Chuchana
  • IRMB, Unité INSERM U1183, Hôpital Saint Eloi, 34295 Montpellier France.
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Jean-loup Lemesre
  • Jean-loup Lemesre
  • Institut de Recherche pour le Développement IRD, UMR 177 « INTERTRYP», Centre IRD de Montpellier, 34000 Montpellier Cedex, France.
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Philippe Vincendeau
  • Philippe Vincendeau
  • Université de Bordeaux, IRD, UMR 177 «INTERTRYP», 33076 Bordeaux France.
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Pierrette Courtois
  • Pierrette Courtois
  • Université de Bordeaux, IRD, UMR 177 «INTERTRYP», 33076 Bordeaux France.
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Sylvie Daulouède
  • Sylvie Daulouède
  • Université de Bordeaux, IRD, UMR 177 «INTERTRYP», 33076 Bordeaux France.
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Philippe Truc
  • Philippe Truc
  • Institut de Recherche pour le Développement IRD, UMR 177 «INTERTRYP», Campus International de Baillarguet, 34398 Montpellier Cedex, France.
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  •  Received: 20 July 2016
  •  Accepted: 25 August 2016
  •  Published: 07 September 2016

Abstract

Antibodies directed against L-tryptophan epitope (WE - W for tryptophan, E for epitope), a constant epitope borne by variant surface glycoproteins (VSG), have been detected in sera of all 152 Human African Trypanosomosis (HAT) patients from Angola. The WE is present in VSG hydrophobic regions of the C terminal domains. In the assay, L-tryptophan was linked to bovine serum albumin (BSA) with glutaraldehyde to synthesize W-G-BSA conjugate which was used in an enzyme-linked immunosorbent assay (ELISA) to detect the antibodies. A significant difference was found between HAT patients and controls confirming previous results obtained with a lower number of patients in Congo. A diagnostic test based on this synthetic epitope, especially in combination with other tests, might improve the HAT diagnostic test in field conditions.

Key words: Tryptophan, enzyme-linked immunosorbent assay (ELISA), human African trypanosomosis, serological diagnostic.