African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Characterization and cloning of TMV resistance gene N homologues from Nicotiana tabacum

  Jun-Shan Gao1*, Yan Meng1, Nobumitsu Sasaki2, Hiromi Kanegae2, Naomi Hayashi2 and Hiroshi Nyunoya2  
  1School of Life Sciences, Anhui Agricultural University, Hefei 230036, China. 2Gene Research Center, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.  
Email: [email protected]

  •  Accepted: 08 October 2010
  •  Published: 22 November 2010

Abstract

Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues andisolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively.These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

 

Key words: N gene, tobacco mosaic virus (TMV), N homologue, Nicotiana tabacum, hypersensitive response.

 

Abbreviation

Abbreviations: RACE, Rapid amplification of cDNA ends; RT-PCR, reverse transcription polymerase chain reaction; MP, movement protein; CP, coat protein;NBS, nucleotide-binding site; LRR, leucine-rich repeat; TIR, Toll-interleukin1 receptor; CC, coiled-coil; HR, hypersensitive response; TMV, Tobacco mosaic virus; Avr, avirulence; cDNA, complementary DNA.