Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues andisolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively.These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.
Key words: N gene, tobacco mosaic virus (TMV), N homologue, Nicotiana tabacum, hypersensitive response.
Abbreviations: RACE, Rapid amplification of cDNA ends; RT-PCR, reverse transcription polymerase chain reaction; MP, movement protein; CP, coat protein;NBS, nucleotide-binding site; LRR, leucine-rich repeat; TIR, Toll-interleukin1 receptor; CC, coiled-coil; HR, hypersensitive response; TMV, Tobacco mosaic virus; Avr, avirulence; cDNA, complementary DNA.
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