African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Full Length Research Paper

Optimized production of phytase by solid-state fermentation using triticale as substrate and source of inducer

Alberto A. Neira-Vielma
  • Alberto A. Neira-Vielma
  • Group of Bioprocesses, Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza S/N. Col. República, CP 25280, Saltillo, Coahuila, México.
  • Google Scholar
Cristobal N. Aguilar
  • Cristobal N. Aguilar
  • Group of Bioprocesses, Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza S/N. Col. República, CP 25280, Saltillo, Coahuila, México.
  • Google Scholar
Anna Ilyina
  • Anna Ilyina
  • Cuerpo Académico de Nanobiociencias, School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza S/N. Col. República, CP 25280, Saltillo, Coahuila, México.
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Juan C. Contreras-Esquivel
  • Juan C. Contreras-Esquivel
  • Group of Bioprocesses, Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza S/N. Col. República, CP 25280, Saltillo, Coahuila, México.
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María das G. Carneiro-da-Cunha
  • María das G. Carneiro-da-Cunha
  • Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP 50.670-420, Recife, PE, Brasil.
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Georgina Michelena-Alvarez
  • Georgina Michelena-Alvarez
  • Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA). Vía Blanca #804 y Carretera Central, Zona Postal 10, Código 11 000, San Miguel del Padrón Ciudad de La Habana, Cuba.
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Jose L. Martínez-Hernandez
  • Jose L. Martínez-Hernandez
  • Cuerpo Académico de Nanobiociencias, School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza S/N. Col. República, CP 25280, Saltillo, Coahuila, México.
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  •  Received: 27 September 2017
  •  Accepted: 06 November 2017
  •  Published: 24 January 2018

Abstract

This study was carried out to evaluate the process of phytase production by Aspergillus niger in solid-state fermentation (SSF) using triticale waste. A waste that currently has no use was reported for this biotechnological process, and is of high impact due to the null use. The process was carried out using an additive free medium, supplemented with only one nitrogen source. Under these conditions, phytase activity of 7.45 U/g dry substrate (DS) was obtained. The process was optimized using different additives such as dextrose, lactose, Tween 80 and potassium chloride. For fermentation maximization, two experimental designs were used: 1) Plackett-Burman design (PBD) and 2) the Box-Behnken design (BBD). PBD was used to evaluate the effect of related variables on the production of phytase, as well as their level of significance in the process, while BBD was used for optimal conditions determination. The process was conducted with Petri dishes and a maximum enzyme activity of 25.8 IU/g DS was obtained. Subsequently, SSF was carried out in a tray to increase the amount of fermented substrate and phytase activity of 23.63 IU/g DS was obtained. The results of this study suggest a minimal decrease (8.4%) in enzyme production with scaling.
 
Key words: Triticale, solid-state fermentation, phytase, Aspergillus niger, optimization, statistical experimental design.