African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Management of Fusarium Wilt using mycolytic enzymes produced by Trichoderma harzianum (Th. Azad)

Mohammad Shahid*
  • Mohammad Shahid*
  • Biocontrol Laboratory, Department of Plant Pathology, CSA University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Mukesh Srivastava
  • Mukesh Srivastava
  • Biocontrol Laboratory, Department of Plant Pathology, CSA University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Sonika Pandey
  • Sonika Pandey
  • Biocontrol Laboratory, Department of Plant Pathology, CSA University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Vipul Kumar
  • Vipul Kumar
  • Biocontrol Laboratory, Department of Plant Pathology, CSA University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Anuradha Singh
  • Anuradha Singh
  • Biocontrol Laboratory, Department of Plant Pathology, CSA University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Shubha Trivedi
  • Shubha Trivedi
  • Biocontrol Laboratory, Department of Plant Pathology, CSA University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Y. K. Srivastava
  • Y. K. Srivastava
  • Biocontrol Laboratory, Department of Plant Pathology, CSA University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Shiv Ram
  • Shiv Ram
  • Biocontrol Laboratory, Department of Plant Pathology, CSA University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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  •  Received: 06 April 2015
  •  Accepted: 14 September 2015
  •  Published: 23 September 2015

Abstract

The main aim of this study was to isolate the best chitinase and glucanase enzyme producing Trichoderma strain to manage the Fusarium wilt disease of Cicer aritenum under in vitro conditions. We also studied the effect of Trichoderma strains on the growth and development of C. aritenum plants. Seven strains of Trichoderma were screened against the Fusarium pathogen to isolate the best biocontrol agent causing maximum inhibition of Fusarium growth. Trichoderma harzianum (Th. Azad) was found to be the best strains among all the tested strains. Trichoderma treated plant exhibited the least disease incidence as compared to control plants. Trichoderma treated plant showed a significant stimulatory effect on all the tested eight parameters as compared to control.

Key words: Trichoderma, antagonistic activity, chitinase, glucanase, Biocontrol agent, phytopathogenic fungi.