African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12392

Full Length Research Paper

In vitro regeneration of Neocarya macrophylla (Sabine) Prance, wild fruit of Niger

Aboubacar Kolafane
  • Aboubacar Kolafane
  • Department of Biology, Faculty of Science and Technology, Abdou Moumouni University of Niamey, P. O. Box, 10.662 Niamey, Niger.
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Sidikou Djermakoye Seyni Ramatou
  • Sidikou Djermakoye Seyni Ramatou
  • Department of Biology, Faculty of Science and Technology, Abdou Moumouni University of Niamey, P. O. Box, 10.662 Niamey, Niger.
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  •  Received: 08 May 2018
  •  Accepted: 18 July 2018
  •  Published: 15 August 2018

Abstract

Neocarya macrophylla is a spontaneous fruit species that constitutes the dominant population of Dallol Bosso in Southwestern Niger. In order to test the in vitro regeneration ability of this species, micro-propagation was applied to micro-cuttings from young almond seedlings. The Murashige and Skoog basal medium was enriched with several concentrations of naphthaleneacetic acid (NAA; 0, 0.5 and 1 mgL-1) and 6-benzylamino purine (BAP; 0, 0.5, 1, 1.5, 2, 2.5 and 3 mgL-1). The first results show that it is possible to obtain vitro-plants by vegetative propagation in vitro even at low doses. Thus, in this test, the overall recovery rate was 67.30%, the lag time was 11 days, and the recovery time was spread over 4 days. The statistical analysis at 5% significance threshold showed that there is no significant difference between the different treatments during the emergence of the buds and the neoformation of the leaves, but at the level of the elongation of the stems, the addition of these growth regulators has significantly impacted the results.

 

Key words: In vitro regeneration, Neocarya macrophylla, micro-cuttings, growth regulators, Niger.