Some creatures are very sensitive to water borne pollutants and can be used as useful bio-indicators of water pollutions. The present study used these bio-indicators to determine the status regarding health of freshwater habitats. The study was carried out to quantify Zn in kidney and liver of Rana tigrina from different aquatic habitats (canal, fish pond and sewage water) by atomic absorption spectrometry (AAS). Liver and kidney tissues of R. tigrina from sewage habitat showed elevated levels of Zn compared to those collected from canal and fish pond water habitats. Hence, it may be concluded that R. tigrina is one of the good bio-indicator species for heavy metals. It can be used to assess the heavy metal load and pollution of aquatic ecosystems.
Key words: Zinc, Rana tigrina, atomic absorption spectrometry (AAS), aquatic habitat.
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