African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

In vitro regeneration of adult trees of Bambusa vulgaris

Aliou NDIAYE, Mamadou Saliou DIALLO, Dame NIANG and Yaye Kène GASSAMA-DIA
Laboratoire de Biotechnologies végétales, Département de Biologie VégétaleUniversité Cheikh Anta DIOP de Dakar.
Email: [email protected]

  •  Accepted: 31 May 2006
  •  Published: 03 July 2006

Abstract

We developed procedures for the regeneration of Bambusa vulgaris using nodal segments from adult plants. Optimal shoot growth was after 16 days cultivation onmodified Murashige and Skoog (MMS) medium supplemented with 2 mg/l of BAP. Elongated shoots of B. vulgaris rooted (45.85%) when cultured in MMS + 20 mg/l IBA. The rooted and acclimated shoots were successfully transferred into the field with 100% of plantlets survival.

 

Key words: Bambusa vulgaris, micropropagation, adult.

Abbreviation

IAA, Indol-3-acetic acid ; IBA : Indol-3-butyric acid. NAA : α-naphthalene acetic acid. BAP : 6-benzylaminopurine. MS : Murashige and Skoog. MMS : modified Murashige and Skoog.