African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12067

Full Length Research Paper

Isolation of low erucic acid-containing genotype of Indian mustard (Brassica juncea Czern. and Coss.) through F1 hybrid anther culture

Amitava Roy# and P. K. Saha*
Department of Botany, Bose Institute, 93/1; A.P.C. Road; Kolkata 700 009, INDIA. #Present address: Department of Botany, Scottish Church College, Kolkata 700 006, INDIA.
Email: [email protected]

  •  Accepted: 05 October 2006
  •  Published: 16 November 2006

Abstract

Reciprocal crosses were done between two cultivars; cv. RJ15 and cv. RLM198 of Indian mustard (Brassica juncea). Anther derived lines designated as Aplants, were raised through anther culture from these F1 hybrid plants. 45% germination was obtained from distinctly shriveled and small Aseeds and grown along with the F2 plants in the same agro-climatic conditions. Subsequently the lines were compared for inheritance pattern between the lines. A normal frequency distribution curve for siliqua per plant was obtained in all the lines reflecting a similar pattern of recombination. Few seeds from the plants of each lines exhibiting high number of siliqua per plant, were isolated for analysis of erucic acid. Three plants in which erucic acid content was lower than the parent cultivars of Ageneration were identified. This showed that contrasting characters could be obtained from Aplants where the traits are oligo or monogenic through anther culture.

 

Key words:  Anther culture, Brassica juncea, Erucic acid, Gas chromatography, Scanning Electron Microscopy (SEM).