African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Antitumor activity of tamoxifen loaded solid lipid nanoparticles on induced mammary tumor gland in Sprague-Dawley rats

Roghaieh Abbasalipourkabir1*, Aref Salehzadeh1 and Rasedee Abdullah2
1Faculty of Medicine, Hamadan University of Medical Science, Iran. 2Faculty of Veterinary Medicine, Universiti Putra Malaysia, Malaysia.
Email: [email protected]

  •  Accepted: 07 September 2010
  •  Published: 25 October 2010

Abstract

An in vivo study was conducted to determine the effect of tamoxifen-loaded solid lipid nanoparticle on LA7 cell-induced rat mammary tumor. After the first tumors appearance, the thirty rats were divided into five groups. Group I served as normal control animal. The other four groups were mammary gland tumor-bearing animals treated with soy oil (Group II), tamoxifen (TAM) dissolved in soy oil (Group III), tamoxifen-loaded solid lipid nanoparticle (Group IV) and solid lipid nanoparticle (SLN) (Group V) dispersed in soy oil. The effect of TAM-loaded SLN in the reduction of tumor size was greater than that of free TAM. The transmission electron microscope analysis showed apoptosis in TAM-loaded SLN and free TAM treatment groups. After treatment, the rats treated with TAM-loaded SLN showed decreases in concentration of serum enzymes. In conclusion, the study suggests that TAM-loaded SLN is very effective for treatment of rat mammary gland tumors and breast cancers.

 

Key words: Mammary tumor gland, Sprague-Dawley rat, tamoxifen loaded solid lipid nanoparticle.

Abbreviation

TAM, Tamoxifen; SLNs, solid lipid nanoparticles; DMEM,Dulbecco’s modified eagle’s medium; FBS, fetal bovine serum; H&E, Hematoxylin-Eosin; TEM, transmission electron microscope; LDH, lactate dehydrogenase; ALP,alkaline phosphatise; ALT, alanine aminotransferase; TAM-SLN, tamoxifen loaded solid lipid nanoparticles.