African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Full Length Research Paper

Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil

Milagre A. Pele
  • Milagre A. Pele
  • Biological Sciences Post-Graduation Program, Federal University of Pernambuco, 50670-420 Recife-PE, Brazil.
  • Google Scholar
Dayana Montero-Rodriguez
  • Dayana Montero-Rodriguez
  • Biological Sciences Post-Graduation Program, Federal University of Pernambuco, 50670-420 Recife-PE, Brazil.
  • Google Scholar
Daylin Rubio-Ribeaux
  • Daylin Rubio-Ribeaux
  • Biological Sciences Post-Graduation Program, Federal University of Pernambuco, 50670-420 Recife-PE, Brazil.
  • Google Scholar
Adriana F. Souza
  • Adriana F. Souza
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife-PE, Pernambuco, Brazil.
  • Google Scholar
Marcos A. C. Luna
  • Marcos A. C. Luna
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife-PE, Pernambuco, Brazil.
  • Google Scholar
Michele F. Santiago
  • Michele F. Santiago
  • Agronomic Institute of Pernambuco (IPA), 50761-000 Recife-PE, Brazil.
  • Google Scholar
Rosileide F. S. Andrade
  • Rosileide F. S. Andrade
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife-PE, Pernambuco, Brazil.
  • Google Scholar
Thayse A. Lima e Silva
  • Thayse A. Lima e Silva
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife-PE, Pernambuco, Brazil.
  • Google Scholar
André L. C. M. A. Santiago
  • André L. C. M. A. Santiago
  • Department of Mycology, Federal University of Pernambuco, 50670-901 Recife-PE, Brazil.
  • Google Scholar
Galba M. Campos-Takaki
  • Galba M. Campos-Takaki
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife-PE, Pernambuco, Brazil.
  • Google Scholar


  •  Received: 05 September 2017
  •  Accepted: 18 January 2018
  •  Published: 07 February 2018

Abstract

This study screened four Rhizopus species as biosurfactant producers using different markers. First of all, Rhizopus spp. UCP 1607 was identified as Rhizopus arrhizus by morphological and molecular methods. The production of biosurfactant/bioemulsifier was investigated by submerged fermentation using soybean post-frying oil (5% v/v) and sodium glutamate (1% w/v) medium. The primary markers’ hemolysis and parafilm M tests showed that R. arrhizus UCP1607 strain exhibited higher hemolytic activity (49 mm of clear zone) on sheep blood agar and a larger drop diameter (12 mm) on parafilm hydrophobic surface. The experimental results showed the most promising biosurfactant production by R. arrhizus UCP 1607 strain led to a reduction of surface tension (31.8 mN/m) and the diameter of the oil-spreading covered an area of 66.4 cm2. The strains Rhizopus microsporus var. chinensis UCP1296, R. microsporus var. microsporus UCP1304, and R. arrhizus UCP1607 were capable of forming stable emulsions corresponding to 91.7, 94.8, and 82.6%, respectively in crude oil.

 

Key words: Tensio-active agent, bioemulsifier, screening of Rhizopus strains, submerged fermentation.