African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12236

Full Length Research Paper

Effects of 1.84 GHz radio-frequency electromagnetic field on sperm maturation in epididymis micro-environment

Guangzhou An*
  • Guangzhou An*
  • Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an 710032, China.
  • Google Scholar
Jing Li
  • Jing Li
  • Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an 710032, China.
  • Google Scholar
Shenglong Xu
  • Shenglong Xu
  • Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an 710032, China.
  • Google Scholar
Tao Zhao
  • Tao Zhao
  • Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an 710032, China.
  • Google Scholar
Kangchu Li
  • Kangchu Li
  • Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an 710032, China.
  • Google Scholar
Jiajin Lin
  • Jiajin Lin
  • Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an 710032, China.
  • Google Scholar
Yan Zhou
  • Yan Zhou
  • Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an 710032, China.
  • Google Scholar
Lihua Zeng
  • Lihua Zeng
  • Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an, 710032, China.
  • Google Scholar
Guozhen Guo
  • Guozhen Guo
  • Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an, 710032, China.
  • Google Scholar
Guirong Ding
  • Guirong Ding
  • Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi’an 710032, China.
  • Google Scholar


  •  Received: 01 August 2015
  •  Accepted: 19 November 2015
  •  Published: 01 June 2016

Abstract

In recent years, intense interest has been drawn to the effects of radio-frequency electromagnetic field (RF-EMF) on reproduction. To explore the effects of RF-EMF on sperm maturation in epididymis microenvironment, 24 male Sprague Dawley (SD) rats were randomly divided into three exposed groups (SAR 1, 2 and 4 W/kg) and one sham group. The rats in exposed group were exposed to 1.84 GHz RF-EMF for 5 days (1 h/day). After that, the rats were anaesthetized immediately and epididymis were taken out. Half of them were fixed in 4% formalin and the others were placed in tissue homogenate. The morphology of sperm and microstructure of epididymis were observed under microscope after hematoxylin-eosin (HE) staining. Expression of Bin1b protein was detected by immunohistochemistry; the level of glutathione (GSH) and enzymes including superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (ALP) and disaccharidase were determined by commercial kits. It was found that, compared with sham group, the sperm morphology and microstructure of epididymis did not change obviously; similarly, there was no significant change in Bin1b protein expression and the levels of GSH, SOD, ACP and ALP in exposure group. These results suggest that 1.84 GHz RF-EMF under this experimental condition could not affect the sperm maturation in epididymis micro-environment of SD rats.

Key words: 1.84 GHz, radio-frequency electromagnetic field (RF-EMF), epididymis, sperm maturation, Bin1b.