African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12236

Full Length Research Paper

Development of a new medium containing date syrup for production of bleomycin by Streptomyces mobaraensis ATCC 15003 using response surface methodology

Hesham H. Radwan1, Fars K. Alanazi2, Ehab I. Taha1, Hamdy A. Dardir3, Ihab M. Moussa4 and Ibrahim A. Alsarra1*
1Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. 2Kayyali Chair for Pharmaceutical Industry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. 3Central Laboratory for Drug and Food Analysis, Ministry of Health, P. O. Box 59082, Riyadh 11525, Saudi Arabia. 4Center of Excellence in Biotechnology Research, King Saud University, P. O. Box 2460, Riyadh 11451, Saudi  Arabia.
Email: [email protected]

  •  Accepted: 16 July 2010
  •  Published: 31 August 2010


A combined statistical approach of orthogonal design and polynomial regression were applied to optimize the composition and concentration of a liquid fermentation medium for the production of bleomycin (BLM) by Streptomyces mobaraensis. Optimal conditions for maximal productivity were determined based on eight parameters at three different levels. The sources of carbon and nitrogen concentration and their interactions with other precursors were found to be statistically significant factors. When date syrup was used as an additional carbon source, higher BLM amount was obtained in comparison to glucose. It was found that the optimum nitrogen source was achieved with the use of soyabean meal. The combined orthogonal design and response surface methodology predicted optimal conditions for production of BLM to be 138 mg dl-1. A confirmatory experiment of the optimal medium composition produced 142 mg dl-1 in the fifth day fermentation at 30°C. The complex medium containing 40 gml-1 date syrup as additional carbon source enhanced the production of BLM by 73%. The combined statistical approach enabled rapid identification and integration of key medium parameters for optimizing secondary metabolite production and could be very useful in pharma-ceutical screening programs.


Key words: Bleomycin, Streptomyces mobaraensis, orthogonal design, medium optimization, date syrup.


BLM, Bleomycin; SS, soluble starch (millet jelly); GC, glucose;SM, soybean meal; CS, corn steep liquor; DS, date Syrup; Zn, Zinc source (ZnSO4.7H2O); Cu, copper source (CuSO4.5H2O); NO, nitrogen source (NaNO3).