African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Full Length Research Paper

Selection of filamentous fungi producing lipases from residual waters of slaughterhouses

Sideney Becker Onofre*
  • Sideney Becker Onofre*
  • Center of Exact and Environmental Sciences - Postgraduate Program in Technology and Innovation Management - PPGTI - Regional Community University of Chapecó - UNOCHAPECÓ - Av. Senador Attílio Fontana, 591-E EFAPI - 89809-000 - Chapecó - Santa Catarina, Brazil.
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Dirceu Abatti
  • Dirceu Abatti
  • Paraná Southwest Education Union - UNISEP - Course in Environmental Engineering - Av. Presidente Kennedy, 2601 - Dois Vizinhos - Paraná - Brazil.
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Douglas Refosco
  • Douglas Refosco
  • Paraná Southwest Education Union - UNISEP - Course in Environmental Engineering - Av. Presidente Kennedy, 2601 - Dois Vizinhos - Paraná - Brazil.
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Amarildo Antonio Tessaro
  • Amarildo Antonio Tessaro
  • Paraná Southwest Education Union - UNISEP - Course in Environmental Engineering - Av. Presidente Kennedy, 2601 - Dois Vizinhos - Paraná - Brazil.
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Jean Alisson Becker Onofre
  • Jean Alisson Becker Onofre
  • Paraná Southwest Education Union - UNISEP - Course in Environmental Engineering - Av. Presidente Kennedy, 2601 - Dois Vizinhos - Paraná - Brazil.
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Alessandra Buss Tessaro
  • Alessandra Buss Tessaro
  • Federal University of Pelotas – UFPel - Postgraduate Program in Materials Science and Engineering. Rua Flores da Cunha, 809 – Pelotas – Rio Grande do Sul, Brazil.
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  •  Received: 25 August 2016
  •  Accepted: 23 December 2016
  •  Published: 01 February 2017

Abstract

The exploration and use of lipases produced by microorganisms are in line with societal concerns since they provide clean development options within the standards of environmental sustainability. The objective of this work was therefore to isolate microorganisms with the potential of producing lipases, which could be used in the waste water treatment systems of slaughterhouse units. Waste water samples were collected from the treatment lagoons of a poultry slaughterhouse. The PCA culture medium was used for the isolation of fungi. The pH was adjusted to 6.5 and autoclaved at 1 atm of pressure, for a time of 30 min. The isolated and purified colonies were evaluated in order to obtain lipase producing strains. The results obtained in this study enabled the conclusion that the waste water produced in slaughterhouse has a high microbial load, highlighting bacteria with 6.8 × 108, filamentous fungi with 3.4 × 104 and yeasts with 2.2 x 102 CFU.ml-1. Out of this microbial population, six species of lipase producing fungi were isolated, with the fungi Aspergillus flavus and Aspergillus terreus being those that obtained the highest enzymatic indices.

Key words: Lipase, fungi, effluent, lipids, enzymes.