African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Full Length Research Paper

Kinetic models and parameters estimation study of biomass and ethanol production from inulin by Pichia caribbica (KC977491)

Mounira Kara Ali*
  • Mounira Kara Ali*
  • Laboratoire de Mycologie, de Biotechnologie et de l'Activité Microbienne (LaMyBAM), Faculté des Sciences de la Nature et de la Vie, Département de Biologie Appliquée, Université Frères Mentouri, Constantine 1-Algeria.
  • Google Scholar
Serge Hiligsmann
  • Serge Hiligsmann
  • Unité de Biotechnologie et Bioprocédés-Service 3BIO -Ecole polytechnique de Bruxelles, Université Libre de Bruxelles (ULB), Belgium.
  • Google Scholar
Nawel Outili
  • Nawel Outili
  • Laboratoire de l'ingénierie des procédés de l'environnement, Faculté de Génie des procédés Université de Constantine 3- Algeria.
  • Google Scholar
Radia Cherfia
  • Radia Cherfia
  • Laboratoire de Mycologie, de Biotechnologie et de l'Activité Microbienne (LaMyBAM), Faculté des Sciences de la Nature et de la Vie, Département de Biologie Appliquée, Université Frères Mentouri, Constantine 1-Algeria.
  • Google Scholar
Noreddine Kacem Chaouche
  • Noreddine Kacem Chaouche
  • Laboratoire de Mycologie, de Biotechnologie et de l'Activité Microbienne (LaMyBAM), Faculté des Sciences de la Nature et de la Vie, Département de Biologie Appliquée, Université Frères Mentouri, Constantine 1-Algeria.
  • Google Scholar


  •  Received: 21 October 2016
  •  Accepted: 11 January 2017
  •  Published: 18 January 2017

Abstract

The growth kinetics and modeling of ethanol production from inulin by Pichia caribbica (KC977491) were studied in a batch system. Unstructured models were proposed using the logistic equation for growth, the Luedeking-Piret equation for ethanol production and modified Leudeking-Piret model for substrate consumption. Kinetic parameters (X0, μm, m, n, p and q) were determined by nonlinear regression, using Levenberg-Marquart method implemented in a Mathcad program. Since the production of ethanol was associated with P. caribbica cell growth, a good agreement between model predictions and experimental data was obtained. Indeed, significant R2 values of 0.91, 0.96, and 0.95 were observed for biomass, ethanol production and substrate consumption, respectively. Furthermore, analysis of variance (ANOVA) was also used to validate the proposed models. According to the obtained results, the predicted kinetic values and experimental data agreed well. Finally, it is possible to predict the development of P. caribbica using these models.

Key words: Pichia caribbica, inulin, bioethanol, numerical simulation.