African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12405

Full Length Research Paper

Effect of crude canine pituitary extract (cCPE) on the in vitro production of progesterone and nuclear maturation of canine oocytes

  Jin-Dan Kang1, Hyo-Sang Lee2, Shuang Liang1, Suo Li1, Yue Lu1, Il-Keun Kong2and Xi-Jun Yin1*        
  1Department of Animal Science, College of Agriculture ,Yanbian University, YanJi 133000, JiLin Province, People’s Republic of China. 2Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University, Suncheon 540-742, JeonNam Province, South Korea.
Email: [email protected]

  •  Accepted: 20 January 2012
  •  Published: 20 March 2012

Abstract

 

We examined the effect of crude canine pituitary extract (cCPE) on the in vitronuclear maturation of canine oocytes and production of progesterone by cumulus cells. cCPE was extracted from canine pituitaries and the concentrations of canine follicle stimulating hormone (FSH) and luteinizing hormone (LH) were determined. Cumulus oocyte complexes (COCs) were harvested from anestrus cycle ovaries and matured in NCSU-37 supplemented with 10% estrus bitch serum, 50 µg/ml gentamycin and 0, 40 or 400 µg/ml cCPE at 38°C in a humidified atmosphere of 5% CO2 for 72 h. The nuclear maturation of the oocytes and the level of progesterone in the culture medium were evaluated. Development to metaphase I (MI) - metaphase II (MII) of canine oocytes in 400 µg/ml cCPE (15.4%) was significantly higher than in 0 and 40 µg/ml cCPE (4.3 and 8.7%), respectively. Treatment with 40 and 400 µg/ml cCPE also generated 0.33 and 0.65 ng/ml progesterone in the culture medium, respectively. Thus, the addition of cCPE to the culture medium promotes the nuclear maturation of canine oocytes and elevates the production of progesterone by cumulus cells.

 

Key words: In vitro maturation, pituitary extract, canine oocyte.

Abbreviation

 cCPE,  Crude canine pituitary extractFSH, follicle stimulating hormoneLH, luteinizing hormoneCOCs, cumulus oocyte complexesMI,metaphase IMII, metaphase II