African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12502

Full Length Research Paper

Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach

Kek Heng Chua1,2*, Boon Pin Kee1 and Kwai Lin Thong3
1Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. 2Centre for Genomics Research, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. 3Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.  
Email: [email protected]

  •  Accepted: 10 August 2009
  •  Published: 15 September 2009

Abstract

A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of approximately 4 kDa was successfully produced and proven by Western blot analysis. This recombinant protein can be used to detect specific anti-Vi antibody produced by typhoid patients. Overall, the His-Vi recombinant protein could serve as a potential diagnostic reagent to detectSalmonella Typhi infection in an individual.

 

Key words: Salmonella enterica serovar Typhi, recombinant protein, Vi mimotope.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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