African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Full Length Research Paper

Quantitative detection of fusion protein rIFN-β-HSA by a sandwich ELISA

Rui-Yu Zhu, Jian-Yong Lei, Lin Qu, Yun Chen and Jian Jin*
Molecular Pharmacology Laboratory, School of Medicine and Pharmaceuticals, Jiangnan University, Wuxi 214122, Peoples Republic of China.
Email: [email protected],[email protected]

  •  Accepted: 30 December 2010
  •  Published: 14 February 2011

Abstract

 

A method that allowed detection and quantification of recombinant fusion protein rIFN-β-HSA in complex mixtures and fermentation media was described. The method was based on a double antibody sandwich ELISA, which was developed by using an anti-IFN monoclonal antibody as capture antibody and an HRP-labeled anti- human serum albumin (HSA) monoclonal antibody as detection antibody. The practical working range was estimated to be 21.01 ng/ml to 672.50 ng/ml and the limit of detection was 13.88 ng/ml. Recoveries ranged from 91.9 to 110.4%, while the intra and inter-assay precisions were <4.86 and <8.08%, respectively.

 

Key words: ELISA, rIFN, human serum albumin (HSA), Pichia pastoris, fermentation.

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