The present study was conducted to optimize the media composition through response surface methodology (RSM) for extracellular polysaccharide (EPS) production in Halobacillus trueperi AJSK strain isolated from the salt pan. Halobacillus trueperi was identified with morphological, biochemical characteristics as well as 16S rRNA gene sequencing method. Production medium was optimized through central composite design. In the present study, the maximum EPS production was achieved under the optimal culture conditions of peptone, glucose, NaCl and MgSO4 and they were 15.50, 22.24, 61.56 and 2.33 g/L, respectively at pH 9.0, 35°C in 72 h. An EPS production of 12.93 (g/L) which was well in agreement with the predicted value was achieved by this optimized procedure. Results of the present study proved that statistical media composition analysis with RSM enhanced the EPS production in Halobacillus trueperi.
Key words: Extracellular polysaccharide, Halobacillus trueperi, response surface methodology, salt pan bacteria.