African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Haematological evaluation of Wistar rats exposed to chronic doses of cadmium, mercury and combined cadmium and mercury

  Hounkpatin, A. S. Y.1*, Edorh, P. A.1,2, Guédénon, P.1, Alimba, C. G.3, Ogunkanmi, A.3, Dougnon, T. V.1, Boni, G.1, Aissi, K. A.1, Montcho, S.1, Loko, F.4, Ouazzani, N.5, Mandi, L.5, Boko, M.1 and Creppy, E. E.6  
  1Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), University of Abomey-Calavi, 03 BP 1463, Cotonou, Benin. 2Departement of Biochemistry and Cellular Biology, University of Abomey-Calavi, 01BP 526 Cotonou, Benin. 3Department of Cell Biology and Genetics, Faculty of Science, University of Lagos, Nigeria 4Polytechnic School of Abomey-Calavi, Research Laboratory in Applied Biology, University of Abomey-Calavi, 01 BP 2009 Cotonou, Benin. 5Laboratory of Hydrobiology, Ecotoxicology and Cleansing, Department of Biology, Faculty of Science Semlalia, University Cadi Ayyad, BP 2390, Marrakech, Morocco. 6Laboratory of Toxicology and Applied Hygiene/UFR of Pharmaceutical Sciences, 146, Road Leo Saignat, 33076 Bordeaux Cedex, France.
Email: [email protected]

  •  Accepted: 10 May 2013
  •  Published: 05 June 2013



Cadmium and mercury present in the environment, cause blood disorders. This study was conducted to evaluate the influence of cadmium, mercury and their combination on hematological parameters of Wistar rats. For this purpose, two different doses of each metal and their combination were administered orally for 28 days to six groups of five rats each. Two groups (A and B) were respectively exposed to CdCl2 (0.25 and 2.5 mg/kg), two other groups (C and D) respectively received HgCl2 (0.12 and 1.2 mg/kg) and the last two groups (E and F) were respectively treated with the combination of these two metals: (0.25 mg/kg    Cd + 0.12 mg/kg Hg) and (2.5 mg/kg Cd + 1.2 mg/kg Hg). The control group (G) received the same volume of distilled water. At the end of exposure, bodies of rats were weighed and the whole blood was collected by retro-orbital sinus method for analysis of hematological parameters. The results of this study show a significant decrease (p<0.05) in white blood cells (WBC) in the lot treated with the combination (0.25 mg/kg Cd + 012 mg/kg Hg) and also indicate a significant decrease (p<0.05) in WBC, red blood cells (RBC), hemoglobin concentration (HGB) and the mean corpuscular hemoglobin concentration (MCHC) with high levels of mercury (2.5 mg/kg) and the combination (2.5 mg/kg Cd + 1.2 mg/kg Hg). An increase in the number of platelet count (PLT) in all intoxicated lots was observed.

Key words: Cadmium, mercury, hematology, blood parameters, rats.


Abbreviations: WBC, White blood cell count; RBC, red blood cells; HGB,hemoglobin concentration; HCT, haematocrit; MCH, mean corpuscular hemoglobin,MCV, volume of mean corpuscular erythrocyte; MCHC, mean corpuscular hemoglobin concentration; PLT, platelet count; LYM, number of lymphocytes; ROS,reactive oxygen species.

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