African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Extended Abstract

Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis

Asunta Mukami
  • Asunta Mukami
  • Department of Biochemistry, South Eastern Kenya University, P. O. Box 170-90200, Kitui, Kenya.
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Alex Ngetich
  • Alex Ngetich
  • Institute of Biotechnology Research, Jomo Kenyatta University of Agriculture Technology, P. O. Box 62000-00200, Nairobi, Kenya.
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Cecilia Mweu
  • Cecilia Mweu
  • Institute of Biotechnology Research, Jomo Kenyatta University of Agriculture Technology, P. O. Box 62000-00200, Nairobi, Kenya.
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Mutemi Muthangya
  • Mutemi Muthangya
  • Department of Biochemistry, South Eastern Kenya University, P. O. Box 170-90200, Kitui, Kenya.
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Richard O. Oduor
  • Richard O. Oduor
  • Department of Biochemistry and Biotechnology, Kenyatta University, P. O. Box 43844-00100, Nairobi, Kenya.
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Mathew Ngugi
  • Mathew Ngugi
  • Department of Biochemistry and Biotechnology, Kenyatta University, P. O. Box 43844-00100, Nairobi, Kenya.
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Wilton Mbinda
  • Wilton Mbinda
  • Department of Biochemistry and Biotechnology, Pwani University, P. O. Box 195-80108, Kilifi, Kenya.
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  •  Received: 19 June 2018
  •  Accepted: 16 July 2018
  •  Published: 18 July 2018

Abstract

A simple and efficient plant regeneration system via direct organogenesis was established in finger millet using in vitro derived shoot apical meristems. Six varieties; GBK-043128, GBK-043094, GBK-043050, GBK-043137, GBK-043122 and GBK-043124 were evaluated. MS medium was used for cotyledonary germination. Maximum number of shoots (84.33%) was observed in variety GBK-043128 while GBK-043094 had the least germination efficiency (62. 67%). Shoot apical meristems from three-day old seedlings were evaluated for their potency of shoot induction on varied 6-benzylaminopurine (BAP) concentrations. Highest shoot induction was observed in medium supplemented with 1.75 mg/l BAP in GBK-043050 (3.00) whereas GBK-043094 (1.28) had the least response in medium supplemented with 1.0 mg/l BAP. To induce rooting, in vitro regenerated plants cultured on MS medium was supplemented with different concentrations of indole-3- acetic acid. The highest response in root induction, with a larger number of roots (10.28), was observed in MS medium supplemented with 4.0 µM IAA. Statistical analysis indicated that plant regeneration response varied greatly among the varieties. In vitro germinated plants were successfully transferred to the greenhouse after hardening, with 300 shoots developing into fertile plants, which were indistinguishable with wild type plants. This plant regeneration system has potential for production of transgenic finger millet crops.

 

Key words: Direct organogenesis, finger millet, root induction, shoot apical meristems.

Abbreviation

BAP, 6-Benzylaminopurine; IAA, Indole-3-acetic acid; MS, Murashige and Skoog basal medium; SAM, shoot apical meristems.