African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Full Length Research Paper

Protection of Lactobacillus acidophilus under in vitro gastrointestinal conditions employing binary microcapsules containing inulin

Rafael E. Gonzalez-Cuello*
  • Rafael E. Gonzalez-Cuello*
  • Facultad de Ingeniería, Programa Ingeniería de Alimentos, Grupo de Investigación en Biotecnologia de Alimentos y Educación (GIBAE), Universidad de Cartagena, Colombia.
  • Google Scholar
Fredy Colpas-Castillo
  • Fredy Colpas-Castillo
  • Facultad de Ciencias Exactas y Naturales. Programa de Química, Carboquimica, Universidad de Cartagena, Colombia.
  • Google Scholar
Arnulfo Tarón-Dunoyer
  • Arnulfo Tarón-Dunoyer
  • Facultad de Ingeniería, Programa Ingeniería de Alimentos, Grupo de Investigación en Biotecnologia de Alimentos y Educación (GIBAE), Universidad de Cartagena, Colombia.
  • Google Scholar


  •  Received: 26 September 2016
  •  Accepted: 14 December 2016
  •  Published: 18 January 2017

Abstract

In this research, microcapsules based on low acyl gellan (LAG) and sodium alginate (SA) containing inulin were developed in order to assess its protective effect on the viability of Lactobacillus acidophilus under in vitro gastrointestinal conditions.The results showed that microencapsulated cells display significantly (P<0.05) higher resistance to simulated gastrointestinal conditions (SGIC) than free cells. Besides, the incorporation of inulin into the wall matrix resulted in improved survival after 5 h incubation in SGIC. These results represent an alternative to vehiculate probiotics in food, especially in solid food due to the size of the microcapsules. Therefore, these microcapsules can contribute to possible industrial applications in the development of new alimentary products.

Key words: Inulin, microcapsules, probiotic, simulated gastrointestinal conditions, sodium alginate, low acyl gellan.