African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Nucellar embryogenesis and plantlet regeneration in monoembryonic and polyembryonic mango (Mangifera indica L.) cultivars

Manish Shukla
  • Manish Shukla
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Khair Tuwair Al-Busaidi
  • Khair Tuwair Al-Busaidi
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Ghaya Sultan Al-Blushi
  • Ghaya Sultan Al-Blushi
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Abdullah Hamed Al-Burashdi
  • Abdullah Hamed Al-Burashdi
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Hamoud Darwish Al-Hasani
  • Hamoud Darwish Al-Hasani
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Mohammed Hamed Al-Jabri
  • Mohammed Hamed Al-Jabri
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Basem Saif AL-Kalbani
  • Basem Saif AL-Kalbani
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar


  •  Received: 05 October 2016
  •  Accepted: 16 December 2016
  •  Published: 28 December 2016

Abstract

Biotic and abiotic stress particularly fungal diseases and salinity are major challenges facing mango cultivations in Oman. Micropropagation technique for multiplying disease resistant and salinity tolerant elite cultivars could be utilized to replace dead and infected plants in mango orchards but standardize in-vitro regeneration protocol via somatic embryogenesis is prerequisite. Nucellar tissues from immature mango fruits of monoembryonic cultivars Alphonso, Amrapali, Dashehari and Zafran, and polyembryonic cultivars Carabao and Turpentine were used as explants to induce somatic embryogenesis plantlets. Gamborg’s B5 macronutrients, Murashige and Skoog micronutrients, iron source, vitamins and organics were used as standard basal media for all types of media used at each stage of somatic embryo development and regeneration. Induction medium 2 containing 2 mg/l 2,4-Dichlorophenoxyacetic acid and 0.5 mg/l 6-Benzylaminopurine were induced highest percentage of primary somatic embryos for Alphonso (22.08%) while induction medium 3 having 1 mg/l 2,4- Dichlorophenoxyacetic acid  with sucrose 60 gm/l and induction medium 1 containing 1 mg/l 2,4- Dichlorophenoxyacetic acid and 0.25 mg/l 6-Benzylaminopurine induced highest percentage of primary somatic embryos in Carabao (29.17%) and Turpentine (42.71%) respectively. Maximum somatic embryo germination were achieved in germination medium 2 containing 0.1 mg/l Indole-3-acetic acid and 0.5 mg/l Gibberellic acid for Alphonso (7.34%) and Turpentine (3.34%) while for Carabao (18.59%) in germination medium 1 which does not contain any plant growth regulators. Germinated plantlets are surviving well in ex-vitro conditions after 4 months of transfer to greenhouse and survival rate of 66.66% for Alphonso, 26.68% for Carabao and 49.16% for Turpentine was obtained.

 

Key words: Mango, nucellar embryogenesis, monoembryonic, polyembryonic, somatic embryo, germination, survival rate.