African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Interaction of IBA and NAA with enzymes in root induction of Crocus sativus (L)

Golandam Sharifi and Hassan Ebrahimzadeh*
Plant Physiology Laboratory Department of Botany, School of Biology, College of Sciences, University of Tehran, P. O. Box: 14155-6455, Tehran, Iran.
Email: [email protected], [email protected]

  •  Accepted: 23 April 2009
  •  Published: 11 January 2010

Abstract

 

Effect of auxin type on some antioxidative enzymes during in vitro root induction of saffron (Crocus sativus L.) was investigated to reveal the variation of activities and isoenzyme banding patterns during root induction of saffron (C. sativus L.). Indole-3- butyric acid (IBA) and naphtalene acetic acid (NAA) at different concentrations were added to the solidified MS or B5 medium. Corm explants of saffron were placed on root inducing media and enzyme levels in explants prior or after adventitious root formation were measured and compared. Percentage of root formation on B5 medium containing 2.46 µM IBA was highest; however the maximum number of root per explant and per cm2 observed on MS medium with 19.6 µM IBA. Significant changes occurred in the activities of these enzymes. Some polymorphisms occurred in patterns of lactate dehydrogenase and esterase during root induction in different treatments.

 

Keywords:  Crocus sativus L., saffron, root formation, IBA, NAA.

Abbreviation

Abbreviations:  IBA, Indole-3- butyric acid; NAA, α-naphthalene acetic acid; NBT,nitro blue tetrazolium; PMS, phenazine metho sulphate; CAT, catalase; PPO,polyphenoloxidase; PRX, peroxidase; EST, esterase; LDH, lactate dehydrogenase and L-DOPA, L-dihydroxy-phenyl-alanine.