African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Effect of 10% sodium ascorbate on Streptococcus mutans adherence to bleached bovine enamel surface

Siavash Savadi Oskoee1, Parnian Alizadeh Oskoee1*, Mohammad Hosein Soroush2, Amir Ahmad Ajami1, Maryam Beheshtirouy1 and Fatemeh Pournaghi-Azar1
1Department of Operative Dentistry, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran. 2Department of microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Email: [email protected]

  •  Accepted: 30 July 2010
  •  Published: 31 August 2010


Sodium ascorbate has been suggested to modify bleaching agents’ side effects especially on composite resin bonding to dental hard tissues. The aim of the present study was to evaluate the effect of 10% sodium ascorbate onStreptococcus mutans adherence to bleached enamel surfaces. Sixty enamel slabs from bovine incisors were used. After sterilization of the intact enamel surfaces with UV light, the specimens were randomly divided into the following treatment groups: (1) immersion in normal saline containing 2%NaN3; (2) bleaching of enamel surfaces with 10% carbamide peroxide; (3) bleaching of enamel surfaces with 10% carbamide peroxide followed by 10% sodium ascorbate treatment. Adherence of S. mutans to enamel surfaces was determined bacteriologically. Data was analyzed using one-way ANOVA and post hoc Tukey tests (P < 0.05).10% sodium ascorbate after bleaching (Group 3) caused a significant increase in surface adherence of S. mutans compared to groups 1 and 2 (P < 0.001). Because of bacterial adherence subsequent to use of sodium ascorbate to bleached enamel caries risk may be increased.


Key words: Sodium ascorbate, Streptococcus mutans, carbamide peroxide.