African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12501

Full Length Research Paper

Cloning and expression of Phanerochaete chrysosporium cellobiohydrolase (cbhI.2)

Howard RL
Microbiology, School of Molecular and Life Sciences, University of Limpopo, P/Bag X1106, Sovenga, 0727, South Africa. 
Email: [email protected]

  •  Accepted: 27 September 2005
  •  Published: 31 October 2005

Abstract

The Phanerochaete chrysosporium ME446 cbhI.2 cDNA was cloned and expressed in Escherichia coli using a pET system. Pulse-labelling revealed the expression of an induced protein of approximately 50-60 kDa that reacted with anti-P. chrysosporium ME446 CBH antibodies. The expressed protein remained undegraded in vivo for 135 min but was biologically inactive in vitro.     

 

Key words: Phanerochaete chrysosporium, cbhI.2, cloning and expression, pulse-labelling.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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