Trimethyltin (TMT) chloride induces limbic system neuro-degeneration, resulting in behavioral alterations including cognitive deficits. The aim of the present study was to investigate the effect of glucogalactan polysaccharide (GA) which purified from the roots of mushroom Agaricus bisporus extract on the Trimethyltin Chloride (TMT) induced neuropathy in rats. Adult male rats (200 ±10 g) were divided into four groups that were fed with basal diet throughout the experiment (28 days). The first group (G I) was control group, the second group (GII) was treated with 300 mg/kg BW GA intraperitoneally (i.p.) daily for 28 day. The third group (GIII) was administered i.p. with 8.0 mg TMT /kg body weight (BW), and the fourth (G IV) was treated like the third group and was injected with GA 300 mg/kg BW daily for 28 day after 48 h of TMT. Many bioactive compounds, which were found in GA did not cause any changes in the second group compared to normal control group. The results revealed that administrated of GA, when given 48 h after TMT treatment exhibited an excellent neuropathy effect, lowering the average of MDA, HSP70, homocystein and the neurotransmitters in brain tissue homogenate and they were markedly reduced by administration of GA to near-normal levels. Neurotransmitters and NO were significantly increased after GA treatment as compared to TMT group. From the comet assay for DNA revealed that, TMT induced statistically significant (P<0.05) increase in the mean value of the tail length and moment. They significantly decreased after GA treatment, suggesting alleviated oxidative stress mediated by TMT. In GA administered TMT-treated rats, they showed improved brain histology and diminished the level of
Key words: Trimethyltin, neuro-degeneration, Agaricus bisporus, glucogalactan
CA1, Cornu ammonis 1; CA3, cornu ammonis 3; AB, water soluble; ABI, water insoluble; CAB, crude of aqua's polysaccharide; CSF, cerebrospinal fluid; AchE, acetylcholine esterase; DA, dopamine; Da,dalton; EtOH, ethanol; EPS: exopolysaccharide; 5-HT, serotonin; MDA, malondialdehyde; NO, nitric oxide; IL-1, interleukin 1; IL-3, interleukin 3, NAOH, sodium hydroxide; GMA, glucogalactan from mushroom (Agaricus bisporus); FTIR, Fourier transforms infrared; KBr, potassium bromide; i.p, intraperitoneal.
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