African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12136

Full Length Research Paper

Comparision of microscopy and RDTs techniques for laboratory detection of malaria

Abdolahi khabisi Samane1*, Hosein Zadeh Nahid1, Shahabi Saaed1, Hushang Khazan1, Haghighi Ali1, Raisii Ahmad2, Edrissian Gholam hosein3 and Abadi alireza4
  1Department of Parasitology and Mycology, Shahid Beheshti Medical University,Tehran, Iran. 2Center of Malaria Control and Prevention, Tehran, Iran. 3School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran. 4Medical Social Department, Shahid Beheshti Medical University, Tehran, Iran.  
Email: [email protected]

  •  Accepted: 25 January 2010
  •  Published: 08 March 2010

Abstract

The development of rapid and specific diagnostic test to identify individuals infected with malaria is important to control severe public health impact of this disease.This study evaluated the ability of rapid malaria diagnostic test (BIOTEC Malaria P.v/P.f rapid device) to detect Plasmodium falciparum and Plasmodium vivax malaria during out break in Chabahar province of Sistan and Balouchestan in Iran. Whole blood samples were obtained from 250 suspected malaria patients. Results from these kit were compared to the reading obtained from 100 fields of Giemsa-stained thick smear blood film.This test is rapid (15 min) for malaria detection. The device is coated with monoclonal antibodies against P. falciparum histidine rich protein-2 (PfHRP2) and enzyme parasite lactate dehydrogenase isomer of P. vivax (PLDH) . A total of 55% samples (138 of 250) were positive by blood films, while 54% samples (136 of 250) were positive by malaria P.v/P.f rapid device. The blood film indicated that 81% (112 of 138) of patients were positive for P.vivax and 19% (26 of 138) of patients were positive for P. falciparum. The malaria P.v/P.f rapid device showed that 79.6% (110 of 138) were positive for P.vivax and 1.4% (2 of 138) were negative. 19% (26 of 138) were positive for P. falciparum. These results demonstrated that the malaria P.v/P.f rapid device had sensitivity of 98.5% and specificity of 100% when compared to traditional blood films. The malaria P.v/P.f rapid device showed an excellent correlation with traditional blood films in identification of both P. vivax and P. falciparum malaria. At present for diagnosis of malaria in endemic area, this kit is rather expensive when compared to microscopy. Neverthless, it is very useful for remote areas where the necessary facilities for microscopic examination are not accessible.

 

Key words: RDTs, PLDH, PfHRP2, malaria.