African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12479

Short Communication

The single-cell gel electrophoresis assay to determine apoptosis induced by siRNA in Colo 320 cells

Huang Hao1*, Fu lei1, Yu Nancai1, Su Wen1 and Qi Xiaojuan2*
1Center of Experimental Medicine, Wuhan First Hospital, Wuhan City 430022, P.R. China. 2Maternal and Child Care Service Centre in Wuhan's Qingshan District, Wuhan City 430022, P.R. China.
Email: [email protected]

  •  Accepted: 05 June 2009
  •  Published: 18 August 2009

Abstract

The aim of the present study was to determine if the pattern of DNA fragmentation determined by the single cell gel electrophoresis assay can be used to determine apoptosis induced by siRNA in Colo 320 cells. When the frequency of appearance of apoptotic cells following was observed over a period of time, there was a significant increase in appearance of apoptosis when using single cell gel electrophoresis assay. The present report demonstrates that the characteristic pattern of apoptotic comets detected by the comet assay corresponds to cells undergoing apoptosis.

 

Key words: the single cell gel electrophoresis assay, Colo 320 cells, apoptosis, comets.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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