African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12254

Full Length Research Paper

In vitro regenerative potentials of the medicinal plant Abutilon indicum (L.) Sweet

Sudarshana Mysore Shankarsingh
  • Sudarshana Mysore Shankarsingh
  • Plant Tissue Culture Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore, Karnataka-570006, India.
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Nissar Ahmad Reshi
  • Nissar Ahmad Reshi
  • Plant Tissue Culture Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore, Karnataka-570006, India.
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Girish Hodiyala Vasanaika
  • Girish Hodiyala Vasanaika
  • Plant Tissue Culture Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore, Karnataka-570006, India.
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  •  Received: 25 July 2015
  •  Accepted: 18 November 2015
  •  Published: 23 March 2016

Abstract

The present study was undertaken to evaluate the most suitable concentration of growth regulators for callus induction and subsequent organogenesis in Abutilon indium (L.) Sweet (Malvaceae) leaf, stem and seed explants. Since the plant has a lot of medicinal importance, it was subjected to culture in vitro. Kinetin for seeds, 2,4-dichlorophenoxyacetic acid (2,4-D), benzyl amino purine (BAP), naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) for leaf as well as BAP and  kinetin for stem were the growth regulators used at different concentrations individually. Results showed that growth regulators at different combinations on subculture resulted in the regeneration of shoots. The excised shoots were transferred to liquid medium for in vitro rhizogenesis and plantlets were acclimatized in the laboratory conditions before they were transferred to garden soil. Histologically, callus sections were with active centres, embryoids and multiple shoot buds. Cytological variations such as multinucleate, chromosome bridges, polyploidy and tracheary elements were noticed in callus cells. The regenerated plants were morphologically and cytologically similar to the in vivo plants.

 

Key words: Embryoids, multiple shoot buds, multinucleate, polyploidy, Malvaceae.