African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Isolation and characterization of arsenite oxidizing Pseudomonas lubricans and its potential use in bioremediation of wastewater

Abdul Rehman*, S. Awais Butt and Shahida Hasnain
Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan  
Email: [email protected]

  •  Accepted: 06 January 2010
  •  Published: 08 March 2010

Abstract

A bacterium, Pseudomonas lubricans, isolated from heavy metal laden industrial wastewater, has been shown to tolerate multiple heavy metals suggesting its importance in bioremediation of industrial effluents. P. lubricans tolerated As(III) up to 3 mg ml-1, Cu2+ up to 0.7 mg ml-1, Hg2+ up to 0.4 mg ml-1, Ni2+ up to 0.4 mg ml-1 and Cr6+ up to 0.5 mg ml-1P. lubricans showed optimum growth at pH 7 while optimum temperature for growth was 30°C. P. lubricans could oxidize As(III) 42% (42 µg mg-1 of protein), 78% (78 µg mg-1 of protein) and 95% (95 µg mg-1 of protein) from the medium after 24, 48 and 72 h of incubation at optimal conditions, respectively. The arsenite oxidizing ability shown by P. lubricans indicates its potential application in biological treatment of wastewaters contaminated with arsenic.

 

Key words: Arsenic, wastewater, Pseudomonas lubricans, bioremediation.

Abbreviation

LB, Luria-bertani; MIC, minimum inhibitory concentration; DCPIP,dichlorophenolindophenol; PMS, phenazine methosilfate.