African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12184

Full Length Research Paper

Morphology, flow cytometry and molecular assessment of ex-vitro grown micropropagated anthurium in comparison with seed germinated plants

Saikat Gantait and Uma Rani Sinniah*
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 07 September 2011
  •  Published: 19 October 2011

Abstract

Micropropagated and seed propagated plantlets of anthurium (Anthurium andreanum Lind. cv. CanCan) were transferred to similar field condition and growth stage. A comparative study was conducted using morphological parameters, ploidy level assessment as well as deoxyribonucleic acid (DNA) fingerprinting using inter simple sequence repeat (ISSR) markers. The in vitro generated anthurium plants exhibited comparable vegetative growth and more sucker production when compared to plants propagated through seeds. No variation in ploidy level wasestablished through flow cytometric study. Genetic assessment through ISSR showed no polymorphism in banding pattern. It was revealed that there was no significant variation between micropropagated and seed propagated plants at ploidy and molecular level assuring the trueness of the micropropagated anthurium clones and their commercial applicability.

 

Key words:  Anthurium andreanum Lind, Ex vitro performance, ISSR, morphological competence, ploidy level.

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