African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Effect of seasonal collection on callus Induction, proliferation and somatic embryogenesis from anther cultures of Heveabrasiliensis MuellArg

Wutthichai Srichuay
  • Wutthichai Srichuay
  • Faculty of Science and Technology, Princess of Naradhiwas University, Narathiwat, Thailand; Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand.
  • Google Scholar
Soontreeya Kalawong
  • Soontreeya Kalawong
  • Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand; Department of Agriculture, Faculty of Science and Technology, BansomdejchaoprayaRajabhat University, Bangkok 10600 Thailand.
  • Google Scholar
Sompong Te-chato*
  • Sompong Te-chato*
  • Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand.
  • Google Scholar


  •  Received: 13 June 2014
  •  Accepted: 04 August 2014
  •  Published: 27 August 2014

Abstract

Hevea brasiliensis that is grown in Thailand and Southeast Asia is a very valuable source of natural rubber. However, few studies have been conducted to evaluate the effects of seasonal effect on plantlet regenera-tion through somatic embryogenesis from anther culture. Thus, this study discussed the effect of season on callus induction subsequent to plantlet regeneration from culturing of anther. Male flowers were aseptically opened and anthers were excised to culture on callus induction medium (CIM) which was MS supplemented with 5% sucrose, 1 mg.L-1 2,4-D, 1 mg.L-1 KN, 1 mg.L-1 NAA. Somatic embryo (SE) developed on MS medium supplemented with 3% sucrose, 0.2 mg.L-1 NAA, 1 mg.L-1 BA, 3 mg.L-1 KN and 0.05 mg.L-1 GA3. This medium is so called embryo induction medium (EIM).Anther collectedfrom different season gave different result in callus induction, proliferation and somatic embryogenesis, healthy anther collected in February 15, 2013 gave the best result in callus induction (86.25%), proliferation (422 mgFW) and somatic embryogenesis (20%). Complete plantlets (15.63%)were obtained on MS medium supplemented with 5% sucrose, 0.5 mg.L-1 KN, 0.2 mg.L-1 IAA, 1 mg.L-1 BA, 0.3 mg.L-1 GA3 and 0.2% phytagel. Seasonal collection plays influence rolein successful somatic embryogenesis in tissue culture of rubber tree.

 

Key words: Hevea brasiliensis, rubber, antherculture, callus induction, seasonal collection, somatic embryogenesis.