African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12405

Full Length Research Paper

Studies on the intiation of callusing and regeneration of plantlets in three different basal media with varied plant growth regulators for the micropropagation of Anthurium scherzeriaum using leaf and spathe as explants  

Jagan Mohan Reddy1* and A. K. Bopaiah2
1Department of Biotechnology, Acharya Institute of Technology, Soladevanahally, Bangalore-560 090, Karnataka, India. 2Department of Botany, St. Joseph’s College, Bangalore-560 001, Karnataka, India.
Email: [email protected]

  •  Accepted: 27 January 2012
  •  Published: 20 March 2012


Anthurium scherzeriaum is one of the most common ornamentals used in floriculture industry. However, there is shortage of planting material supplied to the cultivators. To overcome this problem, many breeders adopted micropropagation techniques. The usage of high dosage of plant growth regulators in the media resulted in mutation and loss of potentiality in the regeneration. An attempt was made to derive a suitable medium and selection of proper explants to avoid undesirable results. Explants like young old leaf segments and spathe were used. Three different basal medias such as Murashige and Skoog (MS), Nitsch and White’ media were used with varying concentration of growth regulators. The obtained results indicated marked improvement in the growth pattern and morphological features of the plants obtained by culturing spathe segments in Nitsch’ medium.
 Key words: Anthurium scherzeriaum, tissue culture, floriculture, ornamental plants.