African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12508

Full Length Research Paper

A new endophytic taxol- and baccatin III-producing fungus isolated from Taxus chinensis var. mairei

Yechun Wang1,2 and Kexuan Tang1*
  1Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai 200240, PR China. 2Current address: Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO 63132, USA.  
Email: [email protected], [email protected]

  •  Accepted: 08 July 2011
  •  Published: 30 November 2011

Abstract

120 endophytic fungi were isolated from the old inner bark of Taxus chinensis var.mairei and only a fungus was detected to produce Taxol and related taxanes inpotato dextrose agar (PDA) medium. The presence of taxol and baccatin III was confirmed by high performance liquid chromatography combined with mass spectrometry (LC-MS) and competitive inhibition enzyme immunoassay (CIEIA). The fungal compound showed cytotoxic activity on liver cancer cell line BEL7402 in vitro. The strain was identified as one of Didymostilbe sp. (designated as DF110) according to its morphological characteristics. The isolation of such fungi provided a promising alternative approach to producing taxol in the near future.

 

Key words: Taxol, baccatin III, endophytic fungus, Didymostilbe sp.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 10 No. 72
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES