African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

Full Length Research Paper

Optimized production of lipase from Bacillus subtilis PCSIRNL-39

Haniya Mazhar
  • Haniya Mazhar
  • Institute of Industrial Biotechnology, Government College University, Lahore-54000, Pakistan.
  • Google Scholar
Naaz Abbas*
  • Naaz Abbas*
  • Food and Biotechnology Research Center, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Ferozpure Road, Lahore-54600, Pakistan.
  • Google Scholar
Sakhawat Ali
  • Sakhawat Ali
  • Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Ferozpure Road Lahore, Pakistan.
  • Google Scholar
Amir Sohail
  • Amir Sohail
  • University of Veterinary and Animal Sciences, Shaykh Abdul Qadir Jilani Road, Lahore 54000, Pakistan.
  • Google Scholar
Zahid Hussain
  • Zahid Hussain
  • Institute of Industrial Biotechnology, Government College University, Lahore-54000, Pakistan.
  • Google Scholar
Syed Shahid Ali
  • Syed Shahid Ali
  • Institute of Molecular Biology and Biotechnology, University of Lahore, 1 KM Defence Road, Lahore, Pakistan.
  • Google Scholar


  •  Received: 31 January 2017
  •  Accepted: 28 March 2017
  •  Published: 10 May 2017

Abstract

Lipases catalyze the hydrolysis of long chain triglycerides. Microbial lipases are receiving much attention because of their industrial potential in the chemical, pharmaceutical, medical, cosmetic, biosurfactant synthesis, leather industries, mutation, agrochemicals and paper manufacturing industries. This article presents the isolation of maximum lipase producing bacteria and the optimization of different conditions for the maximum production of lipase. Bacillus subtilis PCSIRNL-39 shows maximum production of lipase at 45°C with pH 7 using nitrogen source peptone and carbon source sucrose. B. subtilis PCSIRNL-39 showed the best production at 5% inoculum size, while Ca2+ and Mg2+ were found best stimulator for enzyme production during the study. Tween 20 and 80 enhanced better lipase production than other surfactant. The kinetic parameters of Vmax and Km for the lipase were measured to be 101 µM/min.mL and 7.6 mg, respectively.

Key words: Bacillus subtilis, microbial lipase, production, optimization.