African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12254

Full Length Research Paper

Effects of morphine on replication of herpes simplex virus type 1 and 2

Seyed Hamid Reza Monavari* and Mahmoud Shamsi Shahrabadi
Department of Virology, Iran University of Medical sciences, Tehran, Iran.
Email: [email protected]

  •  Accepted: 24 February 2009
  •  Published: 17 May 2010



Several drugs are being used in treatment of HSV (Herpesviridae) infection in human but still introducing an effective safe drug is desirable. We investigated the inhibitory effect of morphine on replication of HSV in vitro. The results indicated that a concentration of up to 200 µg/ml morphine had a limited effect on Vero cell viability. At this concentration, the growth of HSV was inhibited considerably and after the third passage in presence of morphine it was completely eliminated. The presence of viral antigens in infected cells in presence of morphine by immunoflourescent staining showed that after the first passage a small number of infected cells contained viral proteins and at the third passage no cells with viral antigen was observed. This was confirmed by page and immunobloting techniques. Electron microscopy observation in cellular section indicated that there was no virus present in treated cells as compared with control untreated infected cells.


Key words: Morphine, HSV1, 2.