African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

Short Communication

Plasmid profile of Escherichia coli 0157:H7 from apparently healthy animals

Smith SI1*, Aboaba OO2, Odeigha P3, Shodipo K2, Adeyeye JA3, Ibrahim A3, Adebiyi T1, Onibokun H1 and Odunukwe NN1
  1Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, P.M.B. 2013, Yaba, Lagos, Nigeria. 2Department of Microbiology, University of Lagos, Nigeria. 3Department of Cell Genetics, University of Lagos, Nigeria.
Email: [email protected]

  •  Accepted: 22 August 2003
  •  Published: 30 September 2003

Abstract

 

One hundred samples from healthy animals were screened for the presence of enterohaemorrhagic Escherichia coli 0157: H7 and 17 were positive for EHEC 0157:H7 after confirmation using serology kits. Antibiotic susceptibility patterns showed the isolates to be highly susceptible to the various antibiotics screened with a few showing multiple antibiotic resistance. The plasmid profiles revealed that 8/17 (47%) of the animal isolates harboured detectable plasmids ranging in size from 0.564 kb to >23 kb.

 

Key words: Escherichia coli, EHEC, animals, plasmid profile.