African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12479

Short Communication

In vitro culture techniques as a tool of sugarcane bud germination study under salt stress

Ch. B. Gandonou1,2,3, C. Agbangla2, C. Ahanhanzo2, T. Errabii1, M. Idaomar1, J. Abrini1 and N. Skali-Senhaji1*
1Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc. 2Laboratoire de Génétique et des Biotechnologies, Faculté des Sciences et Techniques (FAST/UAC),  01B.P. 526, Cotonou, Bénin. 3Centre Béninois de la Recherche Scientifique et Technique (CBRST), Immeuble Soglo, Maro Militaire, 03B.P. 1665 Cotonou, Bénin.
Email: [email protected]

  •  Accepted: 25 August 2008
  •  Published: 20 October 2008

Abstract

Germination was the first stage confronted to soil salinity and it is important to determine salt effects on this stage. In this study, we reported an in vitro procedure for studying sugarcane bud germination and shoot growth under salt stress with different NaCl concentrations (0, 17, 34, 68 and 102 mM) using cultivar NCo310. Germination percentage of control was about 92% after 8 days. Germination of buds, plant fresh and dry mass decreased with increasing salinity. Data indicated that in vitro culture techniques could be used to evaluate salt stress effects in sugarcane at the germination stage.

 

Key words: in vitro culture, sugarcane, buds germination, salt stress, shoot growth.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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