African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Application of polymeric nanoparticles for controlled release of ethanolic extract of guapeva leaves (Pouteria gardneriana Radlk) against Riphicephalus (Boophilus) microplus through in vitro studies

Priscila Fernanda Pereira Barbosa
  • Priscila Fernanda Pereira Barbosa
  • Goiano Federal Institute, Rio Verde Goiás, Brazil.
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Pablo Peres de Mendonça
  • Pablo Peres de Mendonça
  • Goiano Federal Institute, Rio Verde Goiás, Brazil.
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Rômulo Davi Albuquerque Andrade
  • Rômulo Davi Albuquerque Andrade
  • Federal Institute of Goias, Luizânia,Goiás, Brazil.
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Ana Carolina Ribeiro Aguiar
  • Ana Carolina Ribeiro Aguiar
  • Goiano Federal Institute, Rio Verde Goiás, Brazil.
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Andréa Rodrigues Chaves
  • Andréa Rodrigues Chaves
  • Federal University of Goias, Goiânia,Goiás, Brazil.
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Adilson Ben da Costa
  • Adilson Ben da Costa
  • Santa Cruz do Sul University, Brazil.
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Fabiano Guimarães Silva
  • Fabiano Guimarães Silva
  • Goiano Federal Institute, Rio Verde Goiás, Brazil.
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  •  Received: 19 January 2016
  •  Accepted: 18 November 2016
  •  Published: 07 December 2016

Abstract

The study was designed to evaluate the ethanolic extract acaricide action from Pouteria gardneriana Radlk specie adsorbed in chitosan nanoparticles for controlled release against Riphicephalus (Boophilus) microplus. Leaves of P. gardneriana Radlk were collected, air-dried, ground and extracted with ethanol. Chitosan nanospheres were obtained by the phase inversion method. Conductometric titration, UV-Vis and FTIR analysis were conducted with the materials to evaluate the chitosan anchoring ability. In vitro test was performed using engorged females for each treatment, which consisted of the control, raw extract and three treatments diluted, with increasing concentrations of 0.2, 0.4 and 1%. The most effectiveness extract was for 0.4% concentration by observing reduction in the eggs mass compared to control group. The nanomaterial proved to be able to anchor and release the insecticide gradually in pH between 5 and 6 regions, which makes it feasible for use in cattle, prolonging the exposure time between the tick and acaricide.

Key words: Extracts, nanoparticle, controlled release, tick.