African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12506

Full Length Research Paper

The gene expressions of DNA methylation/demethylation enzymes and cytochrome c oxidase subunit 4 in skeletal muscle of thyroidectomized rats

Shuhong Peng1,2, Ningying Xu1, Liping Huang2, Xiaoheng Gao3, Weifeng Zhu3,Riyue Yu2 and Hongning Liu3*
  1College of Animal Science, Zhejiang University, Hangzhou, 310029, People’s Republic of China. 2Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, People’s Republic of China. 3Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine,Nanchang,330004, People’s Republic of China.
Email: [email protected]

  •  Accepted: 08 December 2010
  •  Published: 31 January 2011

Abstract

 

A decrease in mRNA levels for cytochrome c oxidase (COX) subunits wasobserved in skeletal muscle of hypothyroid rats. However, the precise expression mechanisms of the related genes in hypothyroid state still remain unclear. This study investigated gene expressions of DNA methyltransferases (Dnmts), DNA demethylases and Cox4 in skeletal muscle of thyroidectomized rats. The findingsshowed that, the mRNA levels of genes encoding Dnmts and DNA demethylases were up-regulated while the mRNA levels of Cox4 down-regulated. These resultsimply that, DNA methylation might down-regulate Cox4 expression and induce a compensatory mechanism for avoiding an excessive incorporation of 5-mC into DNA.

 

Key words: Gene expression, Dnmts, Mbd4, Gadd45α, Cox4.

Abbreviation

Abbreviations: COX, Cytochrome c oxidase; Dnmts, DNA methyltransferases;RT-PCR, Real-time polymerase chain reaction amplification.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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