African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12433

Full Length Research Paper

Liver enzyme evaluation and NAT2 polymorphism in patients on anti-tuberculosis and antiretroviral drugs at Jamot Hospital in Yaoundé-Cameroon

Ambassa Axel Cyriaque
  • Ambassa Axel Cyriaque
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Tiani Lionel Ulrich
  • Tiani Lionel Ulrich
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Djuidje Ngounoue Marceline
  • Djuidje Ngounoue Marceline
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Assam Assam Jean Paul
  • Assam Assam Jean Paul
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Andoseh Généviève
  • Andoseh Généviève
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Kamdem Thiomo Diane
  • Kamdem Thiomo Diane
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Tchinda Fossi Cédric
  • Tchinda Fossi Cédric
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Nkah Numfor Leonard
  • Nkah Numfor Leonard
  • Fondation Congolaise pour la Recherche Médicale, Brazaville, Republic of Congo.
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Gonsu Kamga Hortense
  • Gonsu Kamga Hortense
  • Department of Microbiology and Infectious Diseases, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Cameroon.
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kouanfack Charles
  • kouanfack Charles
  • Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
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Pefura Yone Eric Walter
  • Pefura Yone Eric Walter
  • Pneumology Service, Yaoundé Jamot Hospital, Cameroon.
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Ntoumi Francine
  • Ntoumi Francine
  • Fondation Congolaise pour la Recherche Médicale, Brazaville, Republic of Congo.
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Penlap Beng Véronique
  • Penlap Beng Véronique
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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  •  Received: 01 December 2021
  •  Accepted: 21 February 2022
  •  Published: 31 March 2022

Abstract

Hepatotoxicity is reported frequently as an adverse reaction during tuberculosis (TB) and HIV treatment. This study aimed to investigate the incidence of antiretroviral and anti-tuberculosis drug-induced liver enzymes activities variation in TB and TB-HIV co-infected patients at Jamot Hospital in Yaoundé-Cameroon. From April 2018 to May 2019, 336 treatment-naïve TB patients were enrolled. Liver enzymes (AST, ALT, ALP) and total bilirubin were evaluated at baseline and 12 weeks after treatment initiation. Blood was spotted on filter paper for DNA extraction by the chelex method. Standard nested PCR followed by restriction enzyme analysis with KpnI, TaqI, and BamHI to detect NAT2 polymorphisms was performed. TB-HIV co-infection prevalence was 29.46%. There was a significant rise of transaminases (p < 0.05) at baseline in TB-HIV co-infected patients. At 12 weeks, there was a substantial rise of transaminases in TB patients, and total bilirubin in TB-HIV co-infected patients (p < 0.05). The prevalence of slow and fast acetylators was 85.71 and 14.29%, respectively. NAT2*5/5 and NAT2*5/6 genotypes were most represented. Slow acetylating NAT2 phenotype was significantly associated with drug hepatotoxicity (p < 0.05). The prevalence of TB-HIV co-infections remains high, and the rise in transaminases is linked to the slow acetylating NAT2 phenotype.

 

Key words: TB/HIV co-infection, hepatotoxicity, NAT2, Jamot Hospital, Cameroon.