African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12392

Full Length Research Paper

Studies on genetic diversity in poplar (Populus deltoides Bartram ex Marsh.) using morphological and random amplified polymorphic DNA (RAPD) marker

Lakshmi Chaudhary1*, Mukesh Kumar2, M. Kumar3, Anil Sindhu4 and Salil K. Tewari1
  1Department of Genetics and Plant Breeding, Govind Ballabh (G.B.) Pant University of Agriculture and Technology, Pantnagar, Uttranchal 263 145, India. 2Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar (Haryana) 125 004, India. 3Department of Biotechnology, Ambala College of Engineering and Applied Research, Mithapur, Ambala Cantt, Haryana 133 101, India. 4Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal (sonepat) Haryana 131 039, India.
Email: [email protected]

  •  Accepted: 22 March 2012
  •  Published: 22 May 2012

Abstract

 

30 popular poplar clones were studied to assess genetic divergence using morphological growth parameters and random amplified polymorphic DNA (RAPD) markers. Based on Mahalanobis D2 analysis, 15 clusters were formed and clustering pattern indicated presence of substantial genetic diversity among 30 poplar clones. These clones were subjected to RAPD analysis, a total of 374 RAPD loci were detected out of which 20 were monomorphic and 354 were polymorphic. A positive correlation between morphological and RAPD analysis in estimating genetic divergence was found. The present molecular study revealed that genotypes from different geographical region clustered in one group, which signifies occurrence of narrow genetic base in that zone. To promote diversified plantation, a multiculture group comprising of S7C1, G-7, 421-2, 82-35-4, PIP-123, D-123, A-194 and 22-N was found to make a broad genetic base for commercial plantations.

 

Key words: Poplar, clones, D2, random amplified polymorphic DNA (RAPD), DNA, diversity.