African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12506

Short Communication

Molecular detection of Edwardsiella tarda with gyrB gene isolated from pirarucu, Arapaima gigas which is exhibited in an indoor private commercial aquarium

Casiano H. Choresca Jr.1, Dennis K. Gomez2, Sang Phil Shin1, Ji Hyung Kim1, Jee Eun Han1, Jin Woo Jun1 and Se Chang Park1*
  1Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea. 2Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines in the Visayas, Miagao, Iloilo, 5023, Republic of the Philippines.  
Email: [email protected]

  •  Accepted: 03 January 2011
  •  Published: 31 January 2011

Abstract

 

The pirarucu, Arapaima gigas, which was displayed in commercial aquarium was found dead and was submitted for diagnostic examination. Bacteria from different organs of the fish were characterized using Vitek System®2 and showed 98% probability to Edwardsiella tarda. Polymerase chain reaction (PCR) result showed positive for E. tarda gyrB gene. The 16S rRNA gene was identical and exhibited 99% sequence similarity with the other known isolates of E. tarda available in the GenBank. This paper reports the isolation and detection of E. tarda with the gyrBgene in pirarucu, A. gigas, which was exhibited in an indoor private commercial aquarium in Seoul, South Korea.

 

Key words: Arapaima gigas, commercial aquarium, Edwardsiella tarda, gyrB gene, pirarucu.

Abbreviation

Abbreviations: TSA, Tryptic soy agar; PCR, polymerase chain reaction; NCBI,National Center For Biotechnology Information; BLAST, Basic Local Alignment Search Tool.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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