Different combinations of plant growth regulators were tried on in vitro gynogenesis by flower bud and ovary culture for two open pollinated Turkish onion cultivars, “Bayram 1” and “Yakut” and a synthetic population of “OH-1”, which serves as a responsive control for extraction of gynogenic haploids of onion for two years (2011 and 2012). In 2011, modiﬁed Dunstan and Short (BDS) medium supplemented with 1 and 2 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) and their combinations were used. Taking into account the first year results, combinations of 2 mg L-1 2,4-D and 1 and 2 mg L-1 of BAP were used in second year experiment. Auxin (2,4-D) and cytokinin (BAP) combined together induced callus development in two onion cultivars (Bayram 1 and Yakut), but haploid plants were obtained only from “OH-1” with 2 mg L-1 of 2,4-D and 1 mg L-1 of BAP combination. Results showed that genotype is more important factor for inducing in vitro gynogenesis than plant growth regulators.
Key words: Allium cepa L., gynogenesis, haploidy, onion.
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