African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12172

Full Length Research Paper

Enhancing 15N-uptake in maize (Zea mays L.) by native Trichoderma spp. strains in Central Mexico

Vera-Nunez, J. A.
  • Vera-Nunez, J. A.
  • Centro de Investigación y de Estudios Avanzados (Cinvestav)-IPN, Unidad Irapuato, km 9.6 Libramiento Norte, Carretera Irapuato-León, 36824 Irapuato, Guanajuato, México.
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Luna-Martínez, F.
  • Luna-Martínez, F.
  • Centro de Investigación y de Estudios Avanzados (Cinvestav)-IPN, Unidad Irapuato, km 9.6 Libramiento Norte, Carretera Irapuato-León, 36824 Irapuato, Guanajuato, México.
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Barcos-Arias, M. S.
  • Barcos-Arias, M. S.
  • Escuela Superior Politécnica del Litoral (ESPOL), Facultad de Ciencias de la Vida, Centro de Investigaciones Biotecnológicas del Ecuador, Campus Gustavo Galindo, km 30.5 Vía Perimetral, EC090112 Guayaquil, Ecuador.
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Avila-Miranda, M. E.
  • Avila-Miranda, M. E.
  • Instituto Tecnológico de Tlajomulco, km 10 Carretera Tlajomulco-San Miguel Cuyutlán, 46640 Tlajomulco de Zúñiga, Jalisco, México.
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Grageda-Cabrera, O. A.
  • Grageda-Cabrera, O. A.
  • Campo Experimental Bajío (CEBAJ), Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), km 6.5 Carretera Celaya-San Miguel de Allende, 38110 Celaya, Guanajuato, México.
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Pena-Cabriales, J. J.
  • Pena-Cabriales, J. J.
  • Centro de Investigación y de Estudios Avanzados (Cinvestav)-IPN, Unidad Irapuato, km 9.6 Libramiento Norte, Carretera Irapuato-León, 36824 Irapuato, Guanajuato, México.
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  •  Received: 17 November 2017
  •  Accepted: 13 March 2019
  •  Published: 29 May 2019

Abstract

A study was undertaken to evaluate the role of Trichoderma strains on 15N-uptake by maize under greenhouse and field conditions. The Trichoderma strains were isolated from different maize production systems in Guanajuato State, Central Mexico. A total of 39 Trichoderma isolates were obtained, 23 correspond to Trichoderma harzianum (Intra-Genic-Segment). Some colonized the endorhizosphere of maize better than others; however, Trichoderma diversity did not correlate with the maize production system (rainfall vs. irrigation conditions). In greenhouse conditions, biomass production and 15N-uptake were equally variable; maize plants inoculated with selected Trichoderma species (high root colonization) and fertilized with 140 mg N kg-1 soil, showed similar increase in grain yield and 15N-uptake vs. those fertilized with 280 mg N kg-1 soil. Biomass and 15N-uptake directly correlated with the capacity of Trichoderma spp. to colonize the rhizosphere. Under field conditions, the N-fertilizer use efficiency was the highest when maize cv. P30G40 was inoculated with T. harzianum T35 at a N fertilization rate of 180 kg N ha-1 (78%). All measured parameters showed positive effects of inoculation under-scoring the feasibility of inoculants with these fungi based on Trichoderma to increase the N-fertilizer use efficiency applied to maize.

Key words: Biofertilizers, N-fertilizer use efficiency, 15N isotope.