African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Short Communication

Isozyme diversity in Cassia auriculata L.

R. Siva1,2* and K. V. Krishnamurthy2
  1The Institutes for Applied Research, Ben Gurion University of the Negev, Beer Sheva, Israel. 2Department of Plant Science, Bharathidasan University, Tiruchirapplli, Tamil Nadu, India.
Email: [email protected]

  •  Accepted: 16 June 2005
  •  Published: 31 August 2005



Cassia auriculata is considered to be one of the important dye yielding and medicinal plants in India. In the present study seeds from fourteen different localities were collected all over India and nine enzymes were screened by native polyacrylamide gel electrophoresis (PAGE) technique and thirty-four putative loci were totally detected. Cluster and factor analyses indicated that there are two major distinct groups or clusters, and thus, seeds collected from a few different localities are enough to capture the genetic variation held by this species. Also isozyme analysis is a reliable, efficient and effective marker technology for determining genetic variations in C. auriculata


Key words: Genetic diversity, isozyme, Cassia auriculata, dye.