African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12236

Full Length Research Paper

Molecular characterization of potato (Solanum tuberosum L.) genotypes using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers

Onamu R.*
  • Onamu R.*
  • Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Texcoco, Mexico. Km 38.5, Carretera México-Texcoco. 56230. Chapingo, Estado de México.
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Legaria J.
  • Legaria J.
  • Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Texcoco, Mexico. Km 38.5, Carretera México-Texcoco. 56230. Chapingo, Estado de México.
  • Google Scholar
Rodríguez J. L.
  • Rodríguez J. L.
  • Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Texcoco, Mexico. Km 38.5, Carretera México-Texcoco. 56230. Chapingo, Estado de México.
  • Google Scholar
Sahagùn J.
  • Sahagùn J.
  • Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Texcoco, Mexico. Km 38.5, Carretera México-Texcoco. 56230. Chapingo, Estado de México.
  • Google Scholar
Pèrez J.
  • Pèrez J.
  • Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Texcoco, Mexico. Km 38.5, Carretera México-Texcoco. 56230. Chapingo, Estado de México.
  • Google Scholar


  •  Received: 30 August 2011
  •  Accepted: 10 August 2012
  •  Published: 01 June 2016

Abstract

19 random amplified polymorphic DNA (RAPD) and five Inter-simple sequence repeat (ISSR) primers were used to characterize 35 potato accessions originating from Mexico, Europe and U.S.A, with the aim of generating information on the genetic diversity and relationship among the genotypes for better exploitation in breeding programs. A total of 222 and 82 loci were generated by the RAPD and ISSR primers, respectively. Both RAPD and ISSR primers revealed 81.45 and 82.98% polymorphism, respectively. Mantel test showed no correlation between RAPD and ISSR (0.27), RAPD and RAPD + ISSR (0.47) similarity matrices. However, a high level of correlation (0.74) was observed between ISSR and RAPD + ISSR similarity matrices. The ISSR marker was found to be more efficient compared to RAPD marker, thereby influencing more the consensus data. The use of consensus data generated more information related to genetic diversity in potato. RAPD and ISSR markers successfully differentiated between the potato accessions and subgrouped the genotypes based on origin. Information on genetic diversity and relationships will be useful in the selection of parents and mapping studies.

Key words: Solanum tuberosum L., random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), genetic diversity, relationships. 

Abbreviation

ISSR, Inter-simple sequence repeat; Ta, annealing temperature; RFLPs, restriction fragment length polymorphism; PCR, polymerase chain reaction; RAPD, random amplified polymorphic DNA; SSR, simple sequence repeats; AFLP, amplified fragment length polymorphism.