African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Full Length Research Paper

Genetic diversity in quarin clover (T. quartinianum) accessions of Ethiopia using ISSR markers

Tadesse Abate
  • Tadesse Abate
  • Department of Animal and Range Science, Madawalabu University, School of Agriculture, P. O. Box 247, Bale Robe, Ethiopia
  • Google Scholar
Kassahun Tesfaye
  • Kassahun Tesfaye
  • Addis Ababa University, Institute of Biotechnology, P. O. Box; Addis Ababa, Ethiopia
  • Google Scholar

  •  Received: 08 July 2015
  •  Accepted: 26 October 2015
  •  Published: 19 April 2017


Genetic diversity of Trifolium quartinianum accessions from Ethiopia were studied using Inter Simple Sequence Repeat (ISSR) markers. A total of 24 accessions, divided into three populations were used for the present study. DNA was extracted from a bulk of samples using a modified CTAB method. A total of 84 bands were amplified by the four di-nucleotid ISSR primers in the overall experimental materials. Genetic diversity was high at the species level (PPL = 100%, h = 0.29, I = 0.44). Comparison of population-based genetic diversity showed that Gojam population was the most diverse. Analysis of molecular variance (AMOVA) revealed high level of within-population variation with 83.13%. Unweighted pair group method with arithmetic average (UPGMA) and Principal Coordinate (PCO) analysis showed that only accessions of T. quartinianum from Gondar formed separate cluster. The study clearly indicates the presence of variable genotypes with their unique identity that deserve conservation attention. 


Key words:  Genetic diversity; Ethiopia; ISSR markers; T. quartinianum.